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Sequence files

Sequence type

Primer sequences

Computing setting

1. Sequence summary (for single end)

(1) Summarize the single-end sequences.

1. Sequence summary (for paired end)

(1) Summarize the paired-end sequences.


Forward reads

Reverse reads

Download the read couts table

Forward sequence length summary

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Download the forward read couts table

Reverse sequence length summary

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Download the reverse read couts table

1. Sequence summary (for Long read)

(1) Summarize the long reads.


Primer sequences table

Select other and enter your primer sequences if you can't find your primer sequence in this table.

2. Sequence denoising (DADA2) for Single end

(1) Start to denoise.


Sample read count summary

Sample summary table

Download

ASV read count summary

ASV summary table

Download

Sequence Length Statistics

Seven-Number Summary of Sequence Lengths

Sequence table

Download the sequences as a FASTA file

2. Sequence denoising (DADA2) for Paired end

(1) Start to denoise.


Sample read count summary

Sample summary table

Download

ASV read count summary

ASV summary table

Download

Sequence Length Statistics

Seven-Number Summary of Sequence Lengths

Sequence table

Download the sequences as a FASTA file

2. Sequence denoising (DADA2) for Long read

(1) Start to denoise.


Sample read count summary

Sample summary table

Download

ASV read count summary

ASV summary table

Download

Sequence Length Statistics

Seven-Number Summary of Sequence Lengths

Sequence table

Download the sequences as a FASTA file

Primer sequences table

Select other and enter your primer sequences if you can't find your primer sequence in this table.

Database

Select the reference database for taxonomy classification.


Reference sequence filtering

1. Check primers

If incorrect, go to 'Step 1.Sequence summary' to select the correct primer.



2. Filter the reference sequence by length

Computing setting

3. Taxonomy classification

(1) Classify taxonomy


(2) Inspect the taxonomy classification result.


(3) Download the files for the next step.

The taxonomic table The ASVs table The seqs data

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Download Taxonomic table
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Download barplot
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Download Heatmap matrix

Table

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Download Alpha Diversity Table

Boxplot

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Download Alpha Diversity Boxplot

Post hoc analysis

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Download Alpha Diversity statistical result


Beta diversity heatmap (Bray-Curtis)

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Download Beta Diversity distance matrix data

Dimension reduction plot

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Statistical analysis

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Download PERMANOVA table
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Download ANOSIM table
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Download MRPP table

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Download pairwise PERMANOVA table
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Download pairwise ANOSIM table
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Download pairwise MRPP table
Phylogenetic diversity is a measure of diversity that take the genetic distance between species into consideration.



The default value is (number of threads on the system -2).



Faith PD table

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Download Faith PD table

Faith PD boxplot

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Download Faith PD boxplot

Post hoc analysis

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Download Faith PD post hoc result

Heatmap of UniFrac distance

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Download UniFrac distance matrix

Dimension reduction plot of UniFrac distance

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Statistical analysis

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Download PERMANOVA table
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Download ANOSIM table
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Download MRPP table

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Download pairwise PERMANOVA table
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Download pairwise ANOSIM table
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Download pairwise MRPP table
ANCOM (Analysis of composition of microbiomes) is used for comparing the composition of microbiomes in two or more populations.



Summary


Function annotation table

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Download the function annotation table

Function barplot

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Download the function barplot
MOCHI Installation

We provide MOCHI local version to let users use MOCHI on their own server. Follow the below steps to install the local version.

docker-compose.yml


Demo

1. Sequence summary (for single end)

2. Sequence denoising (DADA2) for Single end


Sample read count summary

Sample summary table

Download

ASV read count summary

ASV summary table

Download

Sequence Length Statistics

Seven-Number Summary of Sequence Lengths

Sequence table

Download the sequences as a FASTA file

3. Taxonomy classification


Inspect the taxonomy classification result.


(3) Download the files for the next step.

The taxonomic table The ASVs table The seqs data
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Download Taxonomic table
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Download barplot
Loading...
Download Heatmap matrix

Table

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Download Alpha Diversity Table

Boxplot

Loading...
Download Alpha Diversity Boxplot

Post hoc analysis

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Download Alpha Diversity statistical result


Beta diversity heatmap (Bray-Curtis)

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Download Beta Diversity distance matrix data

Dimension reduction plot

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Statistical analysis

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Download PERMANOVA table
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Download ANOSIM table
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Download MRPP table

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Download pairwise PERMANOVA table
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Download pairwise ANOSIM table
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Download pairwise MRPP table

Faith PD table

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Download Faith PD table

Faith PD boxplot

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Download Faith PD boxplot

Post hoc analysis

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Download Faith PD post hoc result

Heatmap of UniFrac distance

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Download UniFrac distance matrix

Dimension reduction plot of UniFrac distance

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Statistical analysis

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Download PERMANOVA table
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Download ANOSIM table
Loading...
Download MRPP table

Loading...
Download pairwise PERMANOVA table
Loading...
Download pairwise ANOSIM table
Loading...
Download pairwise MRPP table
ANCOM (Analysis of composition of microbiomes) is used for comparing the composition of microbiomes in two or more populations.


Summary


Function annotation table

Loading...
Download the function annotation table

Function barplot

Loading...
Download the function barplot

1. Sequence summary (for paired end)


Forward reads

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Reverse reads

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Download the read couts table

Forward sequence length summary

Loading...
Download the forward read couts table

Reverse sequence length summary

Loading...
Download the reverse read couts table

2. Sequence denoising (DADA2) for Paired end


Sample read count summary

Sample summary table

Download

ASV read count summary

ASV summary table

Download

Sequence Length Statistics

Seven-Number Summary of Sequence Lengths

Sequence table

Download the sequences as a FASTA file

1. Sequence summary (for Long read)

2. Sequence denoising (DADA2) for Long read


Sample read count summary

Sample summary table

Download

ASV read count summary

ASV summary table

Download

Sequence Length Statistics

Seven-Number Summary of Sequence Lengths

Sequence table

Download the sequences as a FASTA file