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Sequence files

Please select and upload the sequence files (*.fastq.gz or *.fq.gz)

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Sequence type

Choose the sequence type

Primer sequences

Check this if your sequences are primer-trimmed reads

Choose the forward primer sequence

Choose the reverse primer sequence

Computing setting

Number of threads MOCHI can use

1. Sequence summary (for single end)

(1) Summarize the single-end sequences.

Forward reads

Download the read couts table

Sequence length summary

Download the quality score table

Download

1. Sequence summary (for paired end)

(1) Summarize the paired-end sequences.

Forward reads

1. Sequence summary (for Long read)

(1) Summarize the long reads.

Forward reads

Download the read couts table

Sequence length summary

Download the quality score table

Download

Primer sequences table

Select other and enter your primer sequences if you can't find your primer sequence in this table.

2. Sequence denoising (DADA2) for Single end

(1) Start to denoise.

Sample read count summary

Sample summary table

Download

ASV read count summary

ASV summary table

Download

Sequence Length Statistics

Seven-Number Summary of Sequence Lengths

Sequence table

Download the sequences as a FASTA file

Download the rarefaction plot Download the rarefaction table

Download

2. Sequence denoising (DADA2) for Paired end

(1) Start to denoise.

Sample read count summary

Sample summary table

Download

ASV read count summary

ASV summary table

Download

Sequence Length Statistics

Seven-Number Summary of Sequence Lengths

Sequence table

Download the sequences as a FASTA file

Download the rarefaction plot Download the rarefaction table

Download

2. Sequence denoising (DADA2) for Long read

(1) Start to denoise.

Sample read count summary

Sample summary table

Download

ASV read count summary

ASV summary table

Download

Sequence Length Statistics

Seven-Number Summary of Sequence Lengths

Sequence table

Download the sequences as a FASTA file

Download the rarefaction plot Download the rarefaction table

Download

Primer sequences table

Select other and enter your primer sequences if you can't find your primer sequence in this table.

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Database

Select the reference database for taxonomy classification.

Reference sequence filtering

1. Check primers

If incorrect, go to 'Step 1.Sequence summary' to select the correct primer.

2. Filter the reference sequence by length

Minimum length

Maximum length

Computing setting

Number of threads MOCHI can use

3. Taxonomy classification

(1) Classify taxonomy

(2) Inspect the taxonomy classification result.

Taxonomy result
Log

Download

(3) Download the files for the next step.

The taxonomic table The ASVs table The seqs data

Upload the metadata file

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Choose file format

MOCHI/QIIME2 output (.qza)

Plain text table (.txt)

Upload the taxonomic table file

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Upload the ASV table (Taxon included)

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Function annotation table
Function barplot

Summary

Function annotation table

Download the function annotation table

Function barplot

Choose the group

Download the function barplot

MOCHI Installation

We provide MOCHI local version to let users use MOCHI on their own server. Follow the below steps to install the local version.

docker-compose.yml

1. Sequence summary (for single end)

(1) Summarize the single-end sequences.

Forward reads

Sequence length summary

1. Sequence summary (for paired end)

(1) Summarize the paired-end sequences.

Forward reads

Reverse reads

Forward sequence length summary

Reverse sequence length summary

1. Sequence summary (for Long read)

(1) Summarize the long reads.

Forward reads

Sequence length summary

Primer sequences table

2. Sequence denoising (DADA2) for Single end

(1) Start to denoise.

Sample read count summary

Sample summary table

ASV read count summary

ASV summary table

Sequence Length Statistics

Seven-Number Summary of Sequence Lengths

Sequence table

2. Sequence denoising (DADA2) for Paired end

(1) Start to denoise.

Sample read count summary

Sample summary table

ASV read count summary

ASV summary table

Sequence Length Statistics

Seven-Number Summary of Sequence Lengths

Sequence table

2. Sequence denoising (DADA2) for Long read

(1) Start to denoise.

Sample read count summary

Sample summary table

ASV read count summary

ASV summary table

Sequence Length Statistics

Seven-Number Summary of Sequence Lengths

Sequence table

Primer sequences table

3. Taxonomy classification

(1) Classify taxonomy

(2) Inspect the taxonomy classification result.

(3) Download the files for the next step.

Table

Boxplot

Post hoc analysis

Beta diversity heatmap (Bray-Curtis)

Dimension reduction plot

Statistical analysis

Faith PD table

Faith PD boxplot

Post hoc analysis

Heatmap of UniFrac distance

Dimension reduction plot of UniFrac distance

Statistical analysis

Summary

Function annotation table

Function barplot

1. Sequence summary (for single end)

Forward reads

Sequence length summary

2. Sequence denoising (DADA2) for Single end

Sample read count summary

Sample summary table

ASV read count summary

ASV summary table

Sequence Length Statistics

Seven-Number Summary of Sequence Lengths

Sequence table

3. Taxonomy classification

Inspect the taxonomy classification result.

(3) Download the files for the next step.

Table

Boxplot

Post hoc analysis

Beta diversity heatmap (Bray-Curtis)